The Histochemical Localization of Triphosphopyridine Nucleotide Diaphorase

The Histochemical Localization of Triphosphopyridine Nucleotide Diaphorase

A histochemical method is described for the localization of triphosphopyridine nucleotide diaphorase using a recently synthesized tetrazolium salt (Nitro-BT). By virtue of the favorable histochemical properties of this reagent, it has been possible to demonstrate that whereas DPN diaphorase is usually restricted to the mitochondria, the TPN diaphorase activity of corresponding cells was distrib...

Triphosphopyridine Nucleotide Diaphorase from Wheat Germ.

11. KREBS, H. A. and HENSELEIT, K. Untersuchungen iiber die harnstoffbildung im tierkorper. Zeit. physiol. Chem. 210: 33. 1932. 12. McELROY, W. D. and GLASS, H. B. Amino acid Metabolism. Pp. 300-320. Johns Hopkins Press, Baltimore 1955. 13. MUELLER, G. C., QUINN, E. M. and RUECKERT, R. R. The formation of 1-phosphoerythrulose-4-C" by homogenates of Swiss chard leaves. Arch. Biochem. Biophys. 55...

A Histochemical Method for the Demonstration of Diphosphopyridine Nucleotide Diaphorase

The present investigation concerning the histochemical demonstration of DPN diaphorase follows the development of a new reagent, Nitro-BT, which has already been used successfully for the cytochemical localization of the succinic dehydrogenase system. The most consistently favorable results were obtained with the lactate-lactic dehydrogenase system buffered at pH 7.4. Using sections of rat kidn...

The measurement of triphosphopyridine nucleotide and reduced triphosphopyridine nucleotide and the role of hemoglobin in producing erroneous triphosphopyridine nucleotide values.

A method is described for determining TPN+ and TPNH in a single tissue homogenate made in dilute NaOH at 0’. One portion, for TPN+, is acidified after adding ascorbic acid to prevent TPNH oxidation. A second portion, for TPNH, is heated (still alkaline) after adding cysteine to prevent oxidation. If desired, a third portion can be analyzed directly without heating for total TPN (TPN+ plus TPNH)...

Preparation of triphosphopyridine nucleotide.